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primary antibodies against drp1  (Proteintech)


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    Proteintech primary antibodies against drp1
    Primary Antibodies Against Drp1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 320 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+drp1/DRP1+(C-terminal)+Antibody/pm41478419-97-17-24
    Average 96 stars, based on 320 article reviews
    primary antibodies against drp1 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Nsun2-mediated m5C methylation of Ncor1 exacerbates sepsis-induced cardiomyopathy by promoting mitochondrial dysfunction.
    Article Snippet: Sepsis-induced cardiomyopathy (SIC) is a severe complication of sepsis characterized by mitochondrial dysfunction and impaired myocardial contractility, yet its molecular pathogenesis remains incompletely understood.. In this study, we demonstrate that excessive mitochondrial fission plays a pivotal role in SIC, contributing to inflammation, oxidative stress, and cardiomyocyte apoptosis.. Pharmacological inhibition of mitochondrial fission using Mdivi-1 alleviated these pathological changes both in vivo and in vitro.

    Incubation:

    Article Title: Nsun2-mediated m5C methylation of Ncor1 exacerbates sepsis-induced cardiomyopathy by promoting mitochondrial dysfunction.
    Article Snippet: Sepsis-induced cardiomyopathy (SIC) is a severe complication of sepsis characterized by mitochondrial dysfunction and impaired myocardial contractility, yet its molecular pathogenesis remains incompletely understood.. In this study, we demonstrate that excessive mitochondrial fission plays a pivotal role in SIC, contributing to inflammation, oxidative stress, and cardiomyocyte apoptosis.. Pharmacological inhibition of mitochondrial fission using Mdivi-1 alleviated these pathological changes both in vivo and in vitro.



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    Intermittent fasting protects against anesthesia/surgery‐induced mitochondrial fission and the reduction of ATP production in the hippocampus. (a) Transmission electron microscopy images of mitochondrial morphology in neurons of mice in ad libitum (AL) and intermittent fasting (IF) groups, exposed to anesthesia/surgery (AS) or control (C) conditions. Scale bar = 2.0 μm. Red arrows indicate mitochondria. (b) Representative western blot images of β‐Actin and VDAC expression in total protein, cytosolic fraction, and mitochondrial fraction of mice. (c) Representative western blot images of <t>DRP1</t> levels in hippocampal mitochondrial fractions of mice ( n = 4 mice/group). (d) Representative western blot images of AMPK/PGC1α signaling pathway in the hippocampus of mice. (e) Quantitative analysis of DRP1 levels in hippocampal mitochondrial fractions and AMPK/PGC1α signaling in hippocampal tissues from immunoblots ( n = 4 mice/group). (f) ATP levels measured in hippocampal tissues of mice ( n = 5 mice/group). (g) Mitochondrial membrane potential levels in hippocampal tissues of mice ( n = 5 mice/group). Results are presented as mean ± standard error of the mean (SEM). * p < 0.05; ** p < 0.01; *** p < 0.001; ns, not significant.
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    Intermittent fasting protects against anesthesia/surgery‐induced mitochondrial fission and the reduction of ATP production in the hippocampus. (a) Transmission electron microscopy images of mitochondrial morphology in neurons of mice in ad libitum (AL) and intermittent fasting (IF) groups, exposed to anesthesia/surgery (AS) or control (C) conditions. Scale bar = 2.0 μm. Red arrows indicate mitochondria. (b) Representative western blot images of β‐Actin and VDAC expression in total protein, cytosolic fraction, and mitochondrial fraction of mice. (c) Representative western blot images of <t>DRP1</t> levels in hippocampal mitochondrial fractions of mice ( n = 4 mice/group). (d) Representative western blot images of AMPK/PGC1α signaling pathway in the hippocampus of mice. (e) Quantitative analysis of DRP1 levels in hippocampal mitochondrial fractions and AMPK/PGC1α signaling in hippocampal tissues from immunoblots ( n = 4 mice/group). (f) ATP levels measured in hippocampal tissues of mice ( n = 5 mice/group). (g) Mitochondrial membrane potential levels in hippocampal tissues of mice ( n = 5 mice/group). Results are presented as mean ± standard error of the mean (SEM). * p < 0.05; ** p < 0.01; *** p < 0.001; ns, not significant.
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    Intermittent fasting protects against anesthesia/surgery‐induced mitochondrial fission and the reduction of ATP production in the hippocampus. (a) Transmission electron microscopy images of mitochondrial morphology in neurons of mice in ad libitum (AL) and intermittent fasting (IF) groups, exposed to anesthesia/surgery (AS) or control (C) conditions. Scale bar = 2.0 μm. Red arrows indicate mitochondria. (b) Representative western blot images of β‐Actin and VDAC expression in total protein, cytosolic fraction, and mitochondrial fraction of mice. (c) Representative western blot images of <t>DRP1</t> levels in hippocampal mitochondrial fractions of mice ( n = 4 mice/group). (d) Representative western blot images of AMPK/PGC1α signaling pathway in the hippocampus of mice. (e) Quantitative analysis of DRP1 levels in hippocampal mitochondrial fractions and AMPK/PGC1α signaling in hippocampal tissues from immunoblots ( n = 4 mice/group). (f) ATP levels measured in hippocampal tissues of mice ( n = 5 mice/group). (g) Mitochondrial membrane potential levels in hippocampal tissues of mice ( n = 5 mice/group). Results are presented as mean ± standard error of the mean (SEM). * p < 0.05; ** p < 0.01; *** p < 0.001; ns, not significant.
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    Intermittent fasting protects against anesthesia/surgery‐induced mitochondrial fission and the reduction of ATP production in the hippocampus. (a) Transmission electron microscopy images of mitochondrial morphology in neurons of mice in ad libitum (AL) and intermittent fasting (IF) groups, exposed to anesthesia/surgery (AS) or control (C) conditions. Scale bar = 2.0 μm. Red arrows indicate mitochondria. (b) Representative western blot images of β‐Actin and VDAC expression in total protein, cytosolic fraction, and mitochondrial fraction of mice. (c) Representative western blot images of <t>DRP1</t> levels in hippocampal mitochondrial fractions of mice ( n = 4 mice/group). (d) Representative western blot images of AMPK/PGC1α signaling pathway in the hippocampus of mice. (e) Quantitative analysis of DRP1 levels in hippocampal mitochondrial fractions and AMPK/PGC1α signaling in hippocampal tissues from immunoblots ( n = 4 mice/group). (f) ATP levels measured in hippocampal tissues of mice ( n = 5 mice/group). (g) Mitochondrial membrane potential levels in hippocampal tissues of mice ( n = 5 mice/group). Results are presented as mean ± standard error of the mean (SEM). * p < 0.05; ** p < 0.01; *** p < 0.001; ns, not significant.
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    Intermittent fasting protects against anesthesia/surgery‐induced mitochondrial fission and the reduction of ATP production in the hippocampus. (a) Transmission electron microscopy images of mitochondrial morphology in neurons of mice in ad libitum (AL) and intermittent fasting (IF) groups, exposed to anesthesia/surgery (AS) or control (C) conditions. Scale bar = 2.0 μm. Red arrows indicate mitochondria. (b) Representative western blot images of β‐Actin and VDAC expression in total protein, cytosolic fraction, and mitochondrial fraction of mice. (c) Representative western blot images of <t>DRP1</t> levels in hippocampal mitochondrial fractions of mice ( n = 4 mice/group). (d) Representative western blot images of AMPK/PGC1α signaling pathway in the hippocampus of mice. (e) Quantitative analysis of DRP1 levels in hippocampal mitochondrial fractions and AMPK/PGC1α signaling in hippocampal tissues from immunoblots ( n = 4 mice/group). (f) ATP levels measured in hippocampal tissues of mice ( n = 5 mice/group). (g) Mitochondrial membrane potential levels in hippocampal tissues of mice ( n = 5 mice/group). Results are presented as mean ± standard error of the mean (SEM). * p < 0.05; ** p < 0.01; *** p < 0.001; ns, not significant.
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    Image Search Results


    Intermittent fasting protects against anesthesia/surgery‐induced mitochondrial fission and the reduction of ATP production in the hippocampus. (a) Transmission electron microscopy images of mitochondrial morphology in neurons of mice in ad libitum (AL) and intermittent fasting (IF) groups, exposed to anesthesia/surgery (AS) or control (C) conditions. Scale bar = 2.0 μm. Red arrows indicate mitochondria. (b) Representative western blot images of β‐Actin and VDAC expression in total protein, cytosolic fraction, and mitochondrial fraction of mice. (c) Representative western blot images of DRP1 levels in hippocampal mitochondrial fractions of mice ( n = 4 mice/group). (d) Representative western blot images of AMPK/PGC1α signaling pathway in the hippocampus of mice. (e) Quantitative analysis of DRP1 levels in hippocampal mitochondrial fractions and AMPK/PGC1α signaling in hippocampal tissues from immunoblots ( n = 4 mice/group). (f) ATP levels measured in hippocampal tissues of mice ( n = 5 mice/group). (g) Mitochondrial membrane potential levels in hippocampal tissues of mice ( n = 5 mice/group). Results are presented as mean ± standard error of the mean (SEM). * p < 0.05; ** p < 0.01; *** p < 0.001; ns, not significant.

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Intermittent Fasting Alleviates Anesthesia/Surgery‐Induced Delirium‐Like Behavior in Aged Mice by Remodeling Gut Microbiota

    doi: 10.1002/cns.70748

    Figure Lengend Snippet: Intermittent fasting protects against anesthesia/surgery‐induced mitochondrial fission and the reduction of ATP production in the hippocampus. (a) Transmission electron microscopy images of mitochondrial morphology in neurons of mice in ad libitum (AL) and intermittent fasting (IF) groups, exposed to anesthesia/surgery (AS) or control (C) conditions. Scale bar = 2.0 μm. Red arrows indicate mitochondria. (b) Representative western blot images of β‐Actin and VDAC expression in total protein, cytosolic fraction, and mitochondrial fraction of mice. (c) Representative western blot images of DRP1 levels in hippocampal mitochondrial fractions of mice ( n = 4 mice/group). (d) Representative western blot images of AMPK/PGC1α signaling pathway in the hippocampus of mice. (e) Quantitative analysis of DRP1 levels in hippocampal mitochondrial fractions and AMPK/PGC1α signaling in hippocampal tissues from immunoblots ( n = 4 mice/group). (f) ATP levels measured in hippocampal tissues of mice ( n = 5 mice/group). (g) Mitochondrial membrane potential levels in hippocampal tissues of mice ( n = 5 mice/group). Results are presented as mean ± standard error of the mean (SEM). * p < 0.05; ** p < 0.01; *** p < 0.001; ns, not significant.

    Article Snippet: We used primary antibodies against DRP1 (1:1000; 8570 T, Cell Signaling, Danvers, MA, USA), AMPKα (1:1000; 25,325, Cell Signaling), and p‐Thr172‐AMPK (1:1000; 2531S, Cell Signaling) and corresponding secondary antibodies conjugated to horseradish peroxidase (1:10,000; 511,203, ZenBio, Chengdu, China).

    Techniques: Transmission Assay, Electron Microscopy, Control, Western Blot, Expressing, Membrane

    Gut microbiota influence hippocampal synaptic ultrastructure and mitochondrial dynamics post‐transplantation. (a) Representative transmission electron microscopy images of synaptic ultrastructure of mice that received fecal microbiota transplant (F) from ad libitum (AL) or intermittent fasting (IF) group mice exposed to anesthesia/surgery (AS). (b) Measurements of postsynaptic density (PSD) length and width ( n = 5 slices/group). Scale bar = 1.0 μm (c, d) Western blot images and quantification of PSD95 levels in the hippocampus of mice ( n = 4 mice/group). (e) Transmission electron microscopy images of mitochondrial morphology of mice. Scale bar = 2.0 μm. Red arrows indicate mitochondria. (f) Quantitative analysis of DRP1 levels in hippocampal mitochondrial fractions and AMPK/PGC1α signaling in hippocampal tissues of mice from immunoblots ( n = 4 mice/group). (g) Representative western blot images of AMPK/PGC1α signaling pathway in the hippocampus of mice. (h) Representative western blot images of DRP1 levels in hippocampal mitochondrial fractions of mice ( n = 4 mice/group). (i) ATP levels measured in hippocampal tissues of mice ( n = 5 mice/group). (j) Mitochondrial membrane potential levels in hippocampal tissues of mice ( n = 5 mice/group). Results are presented as mean ± standard error of the mean (SEM). * p < 0.05; ** p < 0.01; *** p < 0.001; ns, not significant.

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Intermittent Fasting Alleviates Anesthesia/Surgery‐Induced Delirium‐Like Behavior in Aged Mice by Remodeling Gut Microbiota

    doi: 10.1002/cns.70748

    Figure Lengend Snippet: Gut microbiota influence hippocampal synaptic ultrastructure and mitochondrial dynamics post‐transplantation. (a) Representative transmission electron microscopy images of synaptic ultrastructure of mice that received fecal microbiota transplant (F) from ad libitum (AL) or intermittent fasting (IF) group mice exposed to anesthesia/surgery (AS). (b) Measurements of postsynaptic density (PSD) length and width ( n = 5 slices/group). Scale bar = 1.0 μm (c, d) Western blot images and quantification of PSD95 levels in the hippocampus of mice ( n = 4 mice/group). (e) Transmission electron microscopy images of mitochondrial morphology of mice. Scale bar = 2.0 μm. Red arrows indicate mitochondria. (f) Quantitative analysis of DRP1 levels in hippocampal mitochondrial fractions and AMPK/PGC1α signaling in hippocampal tissues of mice from immunoblots ( n = 4 mice/group). (g) Representative western blot images of AMPK/PGC1α signaling pathway in the hippocampus of mice. (h) Representative western blot images of DRP1 levels in hippocampal mitochondrial fractions of mice ( n = 4 mice/group). (i) ATP levels measured in hippocampal tissues of mice ( n = 5 mice/group). (j) Mitochondrial membrane potential levels in hippocampal tissues of mice ( n = 5 mice/group). Results are presented as mean ± standard error of the mean (SEM). * p < 0.05; ** p < 0.01; *** p < 0.001; ns, not significant.

    Article Snippet: We used primary antibodies against DRP1 (1:1000; 8570 T, Cell Signaling, Danvers, MA, USA), AMPKα (1:1000; 25,325, Cell Signaling), and p‐Thr172‐AMPK (1:1000; 2531S, Cell Signaling) and corresponding secondary antibodies conjugated to horseradish peroxidase (1:10,000; 511,203, ZenBio, Chengdu, China).

    Techniques: Transplantation Assay, Transmission Assay, Electron Microscopy, Western Blot, Membrane

    Short‐chain fatty acid supplementation protects hippocampal synaptic integrity and mitochondrial function post‐anesthesia/surgery. (a) Representative transmission electron microscopy images of synaptic ultrastructure of mice that SCFA‐supplemented mice (SCFAs+AS) compared to controls (Con+AS) post‐surgery. Scale bar = 1.0 μm (b) Measurements of postsynaptic density (PSD) length and width ( n = 5 slices/group). (c, d) Western blot images and quantification of PSD95 levels in the hippocampus of mice ( n = 4 mice/group). (e) Transmission electron microscopy images of mitochondrial morphology of mice. Scale bar = 2.0 μm. Red arrows indicate mitochondria. (f, g) Western blot images and quantification of DRP1 levels in hippocampal mitochondrial fractions ( n = 4 mice/group). (h) ATP levels measured in hippocampal tissues of mice ( n = 5 mice/group). (i) Mitochondrial membrane potential levels in hippocampal tissues of mice ( n = 5 mice/group). Results are presented as mean ± standard error of the mean (SEM). * p < 0.05; ** p < 0.01; ns, not significant.

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Intermittent Fasting Alleviates Anesthesia/Surgery‐Induced Delirium‐Like Behavior in Aged Mice by Remodeling Gut Microbiota

    doi: 10.1002/cns.70748

    Figure Lengend Snippet: Short‐chain fatty acid supplementation protects hippocampal synaptic integrity and mitochondrial function post‐anesthesia/surgery. (a) Representative transmission electron microscopy images of synaptic ultrastructure of mice that SCFA‐supplemented mice (SCFAs+AS) compared to controls (Con+AS) post‐surgery. Scale bar = 1.0 μm (b) Measurements of postsynaptic density (PSD) length and width ( n = 5 slices/group). (c, d) Western blot images and quantification of PSD95 levels in the hippocampus of mice ( n = 4 mice/group). (e) Transmission electron microscopy images of mitochondrial morphology of mice. Scale bar = 2.0 μm. Red arrows indicate mitochondria. (f, g) Western blot images and quantification of DRP1 levels in hippocampal mitochondrial fractions ( n = 4 mice/group). (h) ATP levels measured in hippocampal tissues of mice ( n = 5 mice/group). (i) Mitochondrial membrane potential levels in hippocampal tissues of mice ( n = 5 mice/group). Results are presented as mean ± standard error of the mean (SEM). * p < 0.05; ** p < 0.01; ns, not significant.

    Article Snippet: We used primary antibodies against DRP1 (1:1000; 8570 T, Cell Signaling, Danvers, MA, USA), AMPKα (1:1000; 25,325, Cell Signaling), and p‐Thr172‐AMPK (1:1000; 2531S, Cell Signaling) and corresponding secondary antibodies conjugated to horseradish peroxidase (1:10,000; 511,203, ZenBio, Chengdu, China).

    Techniques: Transmission Assay, Electron Microscopy, Western Blot, Membrane